Using a qPCR-based solution, KAPA Library Quantification Kits provide accurate and reliable quantification of libraries prepared for sequencing on Illumina and IonTorrent platforms, across a wide range of library types, concentrations, fragment distributions and GC content. KK5702. 2 $422.18 Add to Order: KK2601. Name: KAPA HiFi HotStart DNA Polymerase with dNTPs. Reactions also included 2.5 mM MgCl2, 0.2 μM of each primer and 10 ng of human genomic DNA. The KAPA HRM FAST Master Mix contains a novel DNA polymerase and buffer system along with the EvaGreen® saturating dye, which is optimized for maximum discrimination between sequence variants. Buy from Supplier : Structured Review. KK2702. Explore our Kapa Biosystems qPCR reagents and discover how you can improve the efficiency of your qPCR workflow and quantify your next breakthrough. Explore our integrated Roche Sample Preparation Solutions, encompassing all the steps required to convert a sample to a … For insect cell expression, human TMEM97 was cloned into the vector pVL1392, and baculovirus was prepared using the BestBac system (Expression Systems) in accordance with the manufacturer’s … *B) The Type IV SNP rs641805 is located on an intron of the dihydropyrimidine dehydrogenase (DPD) gene. 500 nM primers and 110 nM probes were used with the following cycling conditions: 95°C for 30 sec followed by 50 cycles of 95°C for 3 sec, and 60°C for 30 sec. Wilmington, MA 01887. For more information, view the KAPA PROBE FAST qPCR Kits FAQs. KAPA Biosystems: Cat#KK8504: NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) New England BioLabs: Cat#E7335L: Deposited Data: Raw and analyzed data: ... lysis buffer (15 mM DTT, 10 mM Tris-EDTA (Sigma-Aldrich, T9285), 0.30% Triton X-100 (Sigma-Aldrich, T9284), 20 mM KCl (Sigma-Aldrich… These kits contain a ready-to-use master mix for highly sensitive and accurate real-time PCR using sequence-specific fluorogenic probe chemistries, including hydrolysis probes (e.g., TaqMan®), FRET probes, and displacement probes (e.g., molecular beacons). Researchers require PCR reagents that reliably perform when they need them. 07959028001. Ooi et al. Kapa Biosystems PCR reagents are engineered using a method known as directed evolution for optimal performance in … KAPA digestion. Merck KGaA will now also be responsible for distributing the polymerase chain reaction (PCR) and real-time PCR – called qPCR – enzymes Roche snagged from its buyout of enzyme-maker Kapa Biosystems in 2015. 100 pg mouse gDNA was amplified targeting the (A) Isg20 (FAM/BHQ-1), (B) F13a1 (CAL Fluor Orange 560), (C) Camta1 (Quasar 670) and (D) Ube20 (Quasar 705) genes. Merck KGaA, Darmstadt, Germany completed its $17 billion acquisition of Sigma-Aldrich in November 2015, creating a leader in the $125 billion global life science industry. All Rights Reserved. | Privacy. We will never provide your information to a third party. Loaded beads were mixed with a polymerase master mix, KAPA2G Robust HotStart ReadyMix (KAPA Biosystems, Wilmington, MA), KAPA HiFi HotStart ReadyMix (KAPA Biosystems) or Q5 High-Fidelity 2X Master Mix (New England Biolabs), 60-nt primer sequences containing 20-nt amplification primer sequences and 40-nt inverted terminal repeats (ITRs) to be used during bulk suppression PCR … Republic OfCook IslandsCosta RicaCôte D'IvoreCroatiaCubaCyprusCzech RepublicDenmarkDjiboutiDominicaDominican RepublicEcuadorEgyptEl SalvadorEquatorial GuineaEritreaEstoniaEthiopiaFalkland IslandsFaroe IslandsFijiFinlandFranceFrench GuianaFrench PolynesiaFrench Southern Terr.GabonGambiaGeorgiaGermanyGhanaGibraltarGreeceGreenlandGrenadaGuadeloupeGuamGuatemalaGuineaGuinea-BissauGuyanaHaitiHeard/McDonald Isls.HondurasHong KongHungaryIcelandIndiaIndonesiaIranIraqIrelandIsraelItalyJamaicaJapanJordanKazakhstanKenyaKiribatiKorea (North)Korea (South)KuwaitKyrgyzstanLaosLatviaLebanonLesothoLiberiaLibyaLiechtensteinLithuaniaLuxembourgMacauMacedoniaMadagascarMalawiMalaysiaMaldivesMaliMaltaMarshall IslandsMartiniqueMauritaniaMauritiusMayotteMexicoMicronesiaMoldovaMonacoMongoliaMontserratMoroccoMozambiqueMyanmarN.
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